Efficient isolation of human parainfluenza viruses 1 and 3 using MNT‐1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect
Identifieur interne : 000902 ( Main/Exploration ); précédent : 000901; suivant : 000903Efficient isolation of human parainfluenza viruses 1 and 3 using MNT‐1, a human malignant melanoma cell line system that exhibits an apparent cytopathic effect
Auteurs : Ko Sato [Japon] ; Oshi Watanabe [Japon] ; Suguru Ohmiya [Japon] ; Fumiko Chiba [Japon] ; Masahiro Hayashi [Japon] ; Tamio Suzuki [Japon] ; Kazuyoshi Kawakami [Japon] ; Hidekazu Nishimura [Japon]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2016-11.
Abstract
Isolation of human parainfluenza virus (HPIV) serotypes 1 and 3 from clinical specimens is not very efficient because of the lack of a cell culture system capable of inducing CPE. In this study, the utility of a melanoma cell line, MNT‐1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT‐1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT‐1 cell culture system has the potential to improve virus isolation from clinical specimens.
Url:
DOI: 10.1111/1348-0421.12446
Affiliations:
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In this study, the utility of a melanoma cell line, MNT‐1, that allows HPIV growth and displays CPE was demonstrated. In particularly, the efficiency of isolating HPIV1 and HPIV3 using MNT‐1 was greater than for cell lines conventionally used for HPIV isolation. Our demonstrated efficacy of HPIV1 and HPIV3 isolation with apparent CPE using the MNT‐1 cell culture system has the potential to improve virus isolation from clinical specimens.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Sato, Ko" sort="Sato, Ko" uniqKey="Sato K" first="Ko" last="Sato">Ko Sato</name></noRegion><name sortKey="Chiba, Fumiko" sort="Chiba, Fumiko" uniqKey="Chiba F" first="Fumiko" last="Chiba">Fumiko Chiba</name><name sortKey="Hayashi, Masahiro" sort="Hayashi, Masahiro" uniqKey="Hayashi M" first="Masahiro" last="Hayashi">Masahiro Hayashi</name><name sortKey="Kawakami, Kazuyoshi" sort="Kawakami, Kazuyoshi" uniqKey="Kawakami K" first="Kazuyoshi" last="Kawakami">Kazuyoshi Kawakami</name><name sortKey="Nishimura, Hidekazu" sort="Nishimura, Hidekazu" uniqKey="Nishimura H" first="Hidekazu" last="Nishimura">Hidekazu Nishimura</name><name sortKey="Nishimura, Hidekazu" sort="Nishimura, Hidekazu" uniqKey="Nishimura H" first="Hidekazu" last="Nishimura">Hidekazu Nishimura</name><name sortKey="Nishimura, Hidekazu" sort="Nishimura, Hidekazu" uniqKey="Nishimura H" first="Hidekazu" last="Nishimura">Hidekazu Nishimura</name><name sortKey="Ohmiya, Suguru" sort="Ohmiya, Suguru" uniqKey="Ohmiya S" first="Suguru" last="Ohmiya">Suguru Ohmiya</name><name sortKey="Sato, Ko" sort="Sato, Ko" uniqKey="Sato K" first="Ko" last="Sato">Ko Sato</name><name sortKey="Suzuki, Tamio" sort="Suzuki, Tamio" uniqKey="Suzuki T" first="Tamio" last="Suzuki">Tamio Suzuki</name><name sortKey="Watanabe, Oshi" sort="Watanabe, Oshi" uniqKey="Watanabe O" first="Oshi" last="Watanabe">Oshi Watanabe</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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